Remote Focusing for 2-photon Microscopy to Follow Action Potential Propagation Transmurally in Acute Rabbit Cardiac Slices

Astrauskaite, G., Mohanan, S. , Williamson, L., Boland, E., Huethorst, E. , Kinegawa, R., Smith, G. and Muellenbroich, C. (2024) Remote Focusing for 2-photon Microscopy to Follow Action Potential Propagation Transmurally in Acute Rabbit Cardiac Slices. In: SPIE BiOS 2024, San Francisco, CA, USA, 27 January - 1 February 2024, ISBN 9781510669536 (doi: 10.1117/12.3002956)

Abstract

In the heart, action potentials travel through the tissue to orchestrate muscle contraction and make the organ an efficient pump. Scar tissues caused by myocardial infarction impairs electrical conduction. In the rabbit heart, action potentials propagate from the endocardium to the epicardium with a conduction velocity of 30 cm/s. Therefore, a rapid vertical scan is necessary to observe this transmural cardiac conduction at cellular resolution. Here we present an implementation of a versatile remote focusing module, compatible with retrofitting to commercial two-photon microscopes and capable of 0.3 kHz rate axial scanning over the range of 100 μm in cardiac tissue without disturbing the sample or the sample objective. We discuss the necessary optimization to compensate for pulse broadening, power losses and optical aberrations. We demonstrate fast imaging of cardiac cell structure in functionally viable rabbit ventricular slice model. We will apply this system to resolve cardiac electrical signal propagation transmurally in healthy and infarcted hearts.

• Item Type: Conference Proceedings
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Smith, Professor Godfrey and Boland, Dr Erin and Kinegawa, Mr Ryo and Williamson, Lewis and Muellenbroich, Dr Caroline and Astrauskaite, Giedre and Mohanan, Ms Sharika and Huethorst, Dr Eline
• Authors: Astrauskaite, G., Mohanan, S., Williamson, L., Boland, E., Huethorst, E., Kinegawa, R., Smith, G., and Muellenbroich, C.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health; College of Science and Engineering > School of Physics and Astronomy
• ISSN: 1605-7422
• ISBN: 9781510669536
• Copyright Holders: Copyright © 2024 SPIE
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher