Lyons, A. , Zickus, V., Alvarez-Mendoza, R., Triggiani, D., Tamma, V., Westerberg, N. , Tassieri, M. and Faccio, D. (2023) Fluorescence lifetime Hong-Ou-Mandel sensing. Nature Communications, 14, 8005. (doi: 10.1038/s41467-023-43868-x) (PMID:38049423) (PMCID:PMC10696080)
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Abstract
Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the performance of the electronics to 100’s of picoseconds. Here, we demonstrate a fluorescence lifetime measurement technique based on photon-bunching statistics with a resolution that is only dependent on the duration of the reference photon or laser pulse, which can readily reach the 1–0.1 picosecond timescale. A range of fluorescent dyes having lifetimes spanning from 1.6 to 7 picoseconds have been here measured with only ~1 s measurement duration. We corroborate the effectiveness of the technique by measuring the Newtonian viscosity of glycerol/water mixtures by means of a molecular rotor having over an order of magnitude variability in lifetime, thus introducing a new method for contact-free nanorheology. Accessing fluorescence lifetime information at such high temporal resolution opens a doorway for a wide range of fluorescent markers to be adopted for studying yet unexplored fast biological processes, as well as fundamental interactions such as lifetime shortening in resonant plasmonic devices.
| Item Type: | Articles |
|---|---|
| Additional Information: | The authors acknowledge financial support from the Royal Academy of Engineering under the Chairs in Emerging Technology and Research Fellowships schemes, and the U.K. Engineering and Physical Sciences Research Council (Grants No. EP/T002123/1, EP/T00097X/1). DF, AL and MT acknowledge financial support from the U.K. Engineering and Physical Sciences Research Council (Grants No. EP/X035905/1). NW wishes to acknowledge support from the Royal Commission for the Exhibition of 1851. VZ received funding from European Social Fund (project No 09.3.3-LMT-K-712-23-0132) under a grant agreement with the Research Council of Lithuania. VT acknowledges support from the Air Force Office of Scientific Research under award number FA8655-23-1- 7046. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Faccio, Professor Daniele and Tassieri, Dr Manlio and Westerberg, Dr Niclas and Alvarez-Mendoza, Mr Raul and Zickus, Dr Vytautas and Lyons, Dr Ashley |
| Authors: | Lyons, A., Zickus, V., Alvarez-Mendoza, R., Triggiani, D., Tamma, V., Westerberg, N., Tassieri, M., and Faccio, D. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Nature Communications |
| Publisher: | Nature Research |
| ISSN: | 2041-1723 |
| ISSN (Online): | 2041-1723 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Nature Communications 14:8005 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
| Data DOI: | 10.5525/gla.researchdata.1524 |
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