Generation, culture and stimulation of small intestinal murine organoids in parasitology research

Campillo Poveda, M. , Drurey, C. and Maizels, R. M. (2023) Generation, culture and stimulation of small intestinal murine organoids in parasitology research. STAR Protocols, 4(4), 102608. (doi: 10.1016/j.xpro.2023.102608) (PMID:37751353) (PMCID:PMC10520931)

Abstract

Parasitic helminth worms frequently infect the gastrointestinal tract and interact with the intestinal epithelium and specialized cell types within it. Intestinal organoids derived from stem cells that line the intestine represent a transformational technology in the study of epithelial-parasite dialogue. Here, we present a protocol for establishing small intestine organoid cultures and administering parasite products of interest to these cultures. We then describe steps for evaluating their impact by microscopy, flow cytometry, immunohistology, and mRNA gene expression. For complete details on the use and execution of this protocol, please refer to Drurey et al. (2022).1

• Item Type: Articles
• Additional Information: This work was supported by the Wellcome Trust through an Investigator Award to R.M.M. (219530), a Collaborative Grant (211814), and the Wellcome Trust core-funded Wellcome Centre for Integrative Parasitology (Ref: 104111).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Drurey, Claire and Campillo Poveda, Dr Marta and Maizels, Professor Rick
• Authors: Campillo Poveda, M., Drurey, C., and Maizels, R. M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: STAR Protocols
• Publisher: Cell Press
• ISSN: 2666-1667
• ISSN (Online): 2666-1667
• Published Online: 25 September 2023
• Copyright Holders: Crown Copyright © 2023
• First Published: First published in STAR Protocols 4(4):102608
• Publisher Policy: Reproduced under a Creative Commons license

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
302416		Tuft cells, sensors adn effectors in immunity to helminths	Richard Maizels	Wellcome Trust (WELLCOTR)	211814/Z/18/Z	SII - Parasitology
170547		The Wellcome Centre for Molecular Parasitology ( Core Support )	Andrew Waters	Wellcome Trust (WELLCOTR)	104111/Z/14/Z	SII - Parasitology
308411		Molecular and Cellular Interactions in Helminth Infections	Richard Maizels	Wellcome Trust (WELLCOTR)	219530/Z/19/Z	SII - Parasitology