Ritchie, R., Barrett, M. P. , Mottram, J. C. and Myburgh, E. (2020) In vivo bioluminescence imaging to assess compound efficacy against Trypanosoma brucei. In: Michels, P. A.M., Ginger, M. L. and Zilberstein, D. (eds.) Trypanosomatids: Methods and Protocols. Series: Methods in molecular biology (2116). Humana Press: New York, NY, pp. 801-817. ISBN 9781071602935 (doi: 10.1007/978-1-0716-0294-2_48)
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Abstract
Traditional animal models for human African trypanosomiasis rely on detecting Trypanosoma brucei brucei parasitemia in the blood. Testing the efficacy of new compounds in these models is cumbersome because it may take several months after treatment before surviving parasites become detectable in the blood. To expedite compound screening, we have used a Trypanosoma brucei brucei GVR35 strain expressing red-shifted firefly luciferase to monitor parasite distribution in infected mice through noninvasive whole-body bioluminescence imaging. This protocol describes the infection and in vivo bioluminescence imaging of mice to assess compound efficacy against T. brucei during the two characteristic stages of disease, the hemolymphatic phase (stage 1) and the encephalitic or central nervous system phase (stage 2).
Item Type: | Book Sections |
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Keywords: | Bioluminescence, firefly luciferase, in vivo imaging, Trypanosoma brucei brucei. |
Status: | Published |
Glasgow Author(s) Enlighten ID: | Barrett, Professor Michael and Mottram, Professor Jeremy and Myburgh, Dr Elmarie and Ritchie, Mr Ryan |
Authors: | Ritchie, R., Barrett, M. P., Mottram, J. C., and Myburgh, E. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Publisher: | Humana Press |
ISBN: | 9781071602935 |
Published Online: | 28 March 2020 |
Copyright Holders: | Copyright © 2020 The Authors |
First Published: | First published in Trypanosomatids: Methods and Protocols: 801-817 |
Publisher Policy: | Reproduced under a Creative Commons License |
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