Presegmentation procedure generates smooth-sided microfluidic devices: unlocking multiangle imaging for everyone?

Hochstetter, A. (2019) Presegmentation procedure generates smooth-sided microfluidic devices: unlocking multiangle imaging for everyone? ACS Omega, 4(25), pp. 20972-20977. (doi: 10.1021/acsomega.9b02139) (PMID:31867488) (PMCID:PMC6921255)

Abstract

We present a simple procedure to create smooth-sided, transparent polymer-based microfluidic devices by presegmentation with hydrophobized glass slides. We study the hypothesis that the smooth side planes permit rapid multiangle imaging of microfluidic systems in contrast to the turbid side planes that result from cutting the polymer. We compare the compatibility of the entire approach with the conventional widefield microscopy, confocal and 2-photon microscopy, as well as three-dimensional (3D) rendering and discuss limitations and potential applications.

• Item Type: Articles
• Additional Information: The author gratefully acknowledges the funding of the Swiss National Science Foundation (P2BSP2_172033) and Animalfree Research (Switzerland). This project (ID 324) has received funding from the EU-H2020 research and innovation program under the grant agreement No. 654360 having benefited from the access provided by the LundNanoLabs in Sweden within the framework of the NFFA-Europe Transnational Access Activity.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hochstetter, Dr Axel
• Authors: Hochstetter, A.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Omega
• Publisher: American Chemical Society
• ISSN: 2470-1343
• ISSN (Online): 2470-1343
• Copyright Holders: Copyright © 2019 American Chemical Society
• First Published: First published in ACS Omega 4(25):20972-20977
• Publisher Policy: Reproduced under a Creative Commons license