Briggs, E. M., Marques, C. A., Reis-Cunha, J., Black, J., Campbell, S., Damasceno, J. , Bartholomeu, D., Crouch, K. and McCulloch, R. (2020) Next-generation analysis of trypanosomatid genome stability and instability. In: Michels, P. A.M., Ginger, M. L. and Zilberstein, D. (eds.) Trypanosomatids: Methods and Protocols. Series: Methods in Molecular Biology (2116). Springer, pp. 225-262. ISBN 9781071602935 (doi: 10.1007/978-1-0716-0294-2_15)
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Publisher's URL: https://www.springer.com/gp/book/9781071602935
Abstract
Understanding the rate and patterns of genome variation is becoming ever more amenable to whole-genome analysis through advances in DNA sequencing, which may, at least in some circumstances, have supplanted more localized analyses by cellular and genetic approaches. Whole-genome analyses can utilize both short- and long-read sequence technologies. Here we describe how sequence generated by these approaches has been used in trypanosomatids to examine DNA replication dynamics, the accumulation of modified histone H2A due to genome damage, and evaluation of genome variation, focusing on ploidy change.
| Item Type: | Book Sections |
|---|---|
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Damasceno, Dr Jeziel and Black, Dr Jennifer Ann and Briggs, Miss Emma and McCulloch, Professor Richard and Crouch, Dr Kathryn and Campbell, Dr Samantha |
| Authors: | Briggs, E. M., Marques, C. A., Reis-Cunha, J., Black, J., Campbell, S., Damasceno, J., Bartholomeu, D., Crouch, K., and McCulloch, R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Publisher: | Springer |
| ISBN: | 9781071602935 |
| Copyright Holders: | Copyright © The Authors |
| First Published: | First published in Methods in Molecular Biology 2116:225-262 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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