Metabolomics on integrated circuit

Cheah, B. C. , MacDonald, A. I., Barrett, M. P. and Cumming, D. R.S. (2017) Metabolomics on integrated circuit. Procedia Technology, 27, pp. 53-54. (doi: 10.1016/j.protcy.2017.04.025)

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We have demonstrated a chip-based diagnostics tool for the quantification of metabolites, using specific enzymes, to study enzyme kinetics and calculate the Michaelis-Menten constant. An array of 256×256 ion-sensitive field effect transistors (ISFETs) fabricated in a complementary metal oxide semiconductor (CMOS) process is used for this prototype. We have used hexokinase enzyme reaction on the ISFET CMOS chip with glucose concentration in the physiological range of 0.05 mM – 231 mM and successfully studied the enzyme kinetics of hexokinase in detail. This will promote future research towards multiplexing enzyme-based metabolite quantification on a single chip, ultimately opening a pathway towards a personal metabolome machine.

Item Type:Articles
Additional Information:Paper presented at Biosensors 2016, Gothenburg, Sweden, 25-27 May 2016.
Glasgow Author(s) Enlighten ID:Cumming, Professor David and MacDonald, Dr Alasdair and Barrett, Professor Michael and Cheah, Dr Boon Chong
Authors: Cheah, B. C., MacDonald, A. I., Barrett, M. P., and Cumming, D. R.S.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Procedia Technology
ISSN (Online):2212-0173
Published Online:13 July 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Procedia Technology 27: 53-54
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
605821The Multi-Corder: Poly-Sensor TechnologyDavid CummingEngineering and Physical Sciences Research Council (EPSRC)EP/K021966/1ENG - ENGINEERING ELECTRONICS & NANO ENG
690421Glasgow Molecular Pathology (GMP) NodeKarin OienMedical Research Council (MRC)MR/N005813/1ICS - EXPERIMENTAL THERAPEUTICS