Cheah, B. C. , MacDonald, A. I., Barrett, M. P. and Cumming, D. R.S. (2017) Metabolomics on integrated circuit. Procedia Technology, 27, pp. 53-54. (doi: 10.1016/j.protcy.2017.04.025)
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Abstract
We have demonstrated a chip-based diagnostics tool for the quantification of metabolites, using specific enzymes, to study enzyme kinetics and calculate the Michaelis-Menten constant. An array of 256×256 ion-sensitive field effect transistors (ISFETs) fabricated in a complementary metal oxide semiconductor (CMOS) process is used for this prototype. We have used hexokinase enzyme reaction on the ISFET CMOS chip with glucose concentration in the physiological range of 0.05 mM – 231 mM and successfully studied the enzyme kinetics of hexokinase in detail. This will promote future research towards multiplexing enzyme-based metabolite quantification on a single chip, ultimately opening a pathway towards a personal metabolome machine.
| Item Type: | Articles |
|---|---|
| Additional Information: | Paper presented at Biosensors 2016, Gothenburg, Sweden, 25-27 May 2016. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cumming, Professor David and MacDonald, Dr Alasdair and Barrett, Professor Michael and Cheah, Dr Boon Chong |
| Authors: | Cheah, B. C., MacDonald, A. I., Barrett, M. P., and Cumming, D. R.S. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering |
| Journal Name: | Procedia Technology |
| Publisher: | Elsevier |
| ISSN: | 2212-0173 |
| ISSN (Online): | 2212-0173 |
| Published Online: | 13 July 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Procedia Technology 27: 53-54 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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