Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Witkowska McConnell, W. et al. (2021) Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus. Nature Communications, 12, 6994. (doi: 10.1038/s41467-021-27076-z) (PMID:34848705) (PMCID:PMC8632961)

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The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in <40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is manufactured at minimal cost using simple, portable equipment. Further development of the diagnostic test will facilitate linkage between disease diagnosis and treatment, greatly improving patient care pathways and reducing loss to follow-up, so assisting in the global elimination strategy.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Sabir, Dr Suleman and Gunson, Dr Rory and Xu, Gaolian and Jajesniak, Dr Pawel and Thomson, Professor Emma and Garrett, Miss Alice and Cooper, Professor Jonathan and Witkowska McConnell, Dr Weronika and Reboud, Dr Julien and Davis, Dr Chris and Bradley-Stewart, Dr Amanda and Yang, Dr Zhugen
Authors: Witkowska McConnell, W., Davis, C., Sabir, S. R., Garrett, A., Bradley-Stewart, A., Jajesniak, P., Reboud, J., Xu, G., Yang, Z., Gunson, R., Thomson, E. C., and Cooper, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nature Communications
Publisher:Nature Research
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Nature Communications 12:
Publisher Policy:Reproduced under a Creative Commons License
Related URLs:
Data DOI:10.5525/gla.researchdata.1127

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190906EPSRC 2015 DTPMary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/M508056/1Research and Innovation Services
300742Accelerating Precision Diagnostics - Bridging the GapAnna DominiczakMedical Research Council (MRC)MC_PC_16045CAMS - Cardiovascular Science
656341Virus-host interactions in hepatitis C virus infectionJohn McLauchlanMedical Research Council (MRC)MC_UU_12014/1MVLS III - CENTRE FOR VIRUS RESEARCH
169538T-cell mediated evolution of hepatitis C virus during acute infectionEmma ThomsonWellcome Trust (WELLCOTR)102789/Z/13/ZIII-MRC-GU Centre for Virus Research