Graphene field effect transistor biosensors based on aptamer for amyloid-β detection

Salehirozveh, M., Dehghani, P., Zimmermann, M., Roy, V. A.L. and Heidari, H. (2020) Graphene field effect transistor biosensors based on aptamer for amyloid-β detection. IEEE Sensors Journal, 20(21), 12488 - 12494. (doi: 10.1109/JSEN.2020.3000583)

Abstract

The development of cost-efficient, sensitive and specific methods to detect amyloid-beta 42 ( Aβ42 ) biomarkers in cerebrospinal fluid and serum-samples is of considerable interest to enable early and reliable diagnosis of Alzheimer’s disease as a precondition for future disease-modifying therapies. This paper presents a reduced graphene oxide field effect transistor (r-GO FET) for label free ultrasensitive detection of an Aβ42 -biomarker with RNA aptamer. The channel in the device was formed by reduction of graphene oxide nanosheets by self-assembly process. As a result, the interaction between Aβ42 and RNA aptamer on the surface of r-GO channel caused a linear response in the shift of the gate voltage ( VTG ) where the minimum conductivity occurs. The r-GO FET can detect the biomarker in range of 1ng/ml to 1pg/ml at pH 7.4 with high specificity. The developed r-GO FET is a low-cost, highly sensitive and selective method for detecting tiny concentrations of Aβ42 , which would also enable measurements in serum-samples.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Heidari, Professor Hadi
• Authors: Salehirozveh, M., Dehghani, P., Zimmermann, M., Roy, V. A.L., and Heidari, H.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: IEEE Sensors Journal
• Publisher: IEEE
• ISSN: 1530-437X
• ISSN (Online): 1558-1748
• Published Online: 08 June 2020
• Copyright Holders: Copyright © 2020 IEEE
• First Published: First published in IEEE Sensors Journal 20(21):12488-12494
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
304896		EPSRC-IAA: Early Stage Commercialisation of a PET Imaging Agent for the Detection of Cardiovascular Disease and Cancer	Andrew Sutherland	Engineering and Physical Sciences Research Council (EPSRC)	EP/R511705/1	Chemistry
303466		HERMES	Hadi Heidari	European Commission (EC)	824164	ENG - Electronics & Nanoscale Engineering