Graphene field effect transistor biosensors based on aptamer for amyloid-β detection

Salehirozveh, M., Dehghani, P., Zimmermann, M., Roy, V. A.L. and Heidari, H. (2020) Graphene field effect transistor biosensors based on aptamer for amyloid-β detection. IEEE Sensors Journal, 20(21), 12488 - 12494. (doi: 10.1109/JSEN.2020.3000583)

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Abstract

The development of cost-efficient, sensitive and specific methods to detect amyloid-beta 42 ( Aβ42 ) biomarkers in cerebrospinal fluid and serum-samples is of considerable interest to enable early and reliable diagnosis of Alzheimer’s disease as a precondition for future disease-modifying therapies. This paper presents a reduced graphene oxide field effect transistor (r-GO FET) for label free ultrasensitive detection of an Aβ42 -biomarker with RNA aptamer. The channel in the device was formed by reduction of graphene oxide nanosheets by self-assembly process. As a result, the interaction between Aβ42 and RNA aptamer on the surface of r-GO channel caused a linear response in the shift of the gate voltage ( VTG ) where the minimum conductivity occurs. The r-GO FET can detect the biomarker in range of 1ng/ml to 1pg/ml at pH 7.4 with high specificity. The developed r-GO FET is a low-cost, highly sensitive and selective method for detecting tiny concentrations of Aβ42 , which would also enable measurements in serum-samples.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Heidari, Dr Hadi
Authors: Salehirozveh, M., Dehghani, P., Zimmermann, M., Roy, V. A.L., and Heidari, H.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:IEEE Sensors Journal
Publisher:IEEE
ISSN:1530-437X
ISSN (Online):1558-1748
Published Online:08 June 2020
Copyright Holders:Copyright © 2020 IEEE
First Published:First published in IEEE Sensors Journal 20(21):12488-12494
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
304896EPSRC-IAA: Early Stage Commercialisation of a PET Imaging Agent for the Detection of Cardiovascular Disease and CancerAndrew SutherlandEngineering and Physical Sciences Research Council (EPSRC)EP/R511705/1Chemistry
303466HERMESHadi HeidariEuropean Commission (EC)824164ENG - Electronics & Nanoscale Engineering