Reversible DNA micro-patterning using the fluorous effect

Flynn, G. E., Withers, J. M., Macias, G., Sperling, J. R. , Henry, S. L., Cooper, J. M. , Burley, G. A. and Clark, A. W. (2017) Reversible DNA micro-patterning using the fluorous effect. Chemical Communications, 53(21), pp. 3094-3097. (doi:10.1039/C7CC00288B) (PMID:28243661) (PMCID:PMC5358500)

[img]
Preview
Text
136996.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

Abstract

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Henry, Dr Sarah and Clark, Dr Alasdair and Cooper, Professor Jonathan and Burley, Dr Glenn and Sperling, Mr Justin and Macias Sotuela, Dr Gerard and Flynn, Miss Gabriella
Authors: Flynn, G. E., Withers, J. M., Macias, G., Sperling, J. R., Henry, S. L., Cooper, J. M., Burley, G. A., and Clark, A. W.
College/School:College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Chemical Communications
Publisher:Royal Society of Chemistry
ISSN:1359-7345
ISSN (Online):1364-548X
Published Online:23 February 2017
Copyright Holders:Copyright © 2017 The Royal Society of Chemistry
First Published:First published in Chemical Communications 53(21): 3094-3097
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.380

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING
561421Plasmon Enhanced Pyroelectric Nanoscale Trapping and SensingAlasdair ClarkThe Royal Academy of Engineering (RAE)10216/103ENG - BIOMEDICAL ENGINEERING
748271EPSRC- Institutional Sponsorship 2016 - University of GlasgowJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/P51133X/1VPO VICE PRINCIPAL RESEARCH & ENTERPRISE
704111Transformable nanophotonic surfaces: fusing synthetic biology with nano-engineering to create physically reconfigurable optical materialsAlasdair ClarkEngineering & Physical Sciences Research Council (EPSRC)EP/N016874/1ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING
690421Glasgow Molecular Pathology (GMP) NodeKarin OienMedical Research Council (MRC)MR/N005813/1ICS - EXPERIMENTAL THERAPEUTICS