DNA-directed spatial assembly of photosynthetic light-harvesting proteins

Henry, S. L., Withers, J. M., Singh, I., Cooper, J. M. , Clark, A. W. , Burley, G. A. and Cogdell, R. J. (2016) DNA-directed spatial assembly of photosynthetic light-harvesting proteins. Organic and Biomolecular Chemistry, 14(4), pp. 1359-1362. (doi:10.1039/c5ob02351c) (PMID:26660647)

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Abstract

This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.

Item Type:Articles
Additional Information:SLH and RJC were supported by funding from Human Frontier of Science Program. AWC was supported by the Royal Academy of Engineering (grant number 10216/103). This research was supported as a part of the Photosynthetic Antenna Research Centre (PARC), an Energy Frontier Research Centre funded by the DOE, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Henry, Dr Sarah and Cogdell, Professor Richard and Clark, Dr Alasdair and Cooper, Professor Jonathan and Burley, Dr Glenn
Authors: Henry, S. L., Withers, J. M., Singh, I., Cooper, J. M., Clark, A. W., Burley, G. A., and Cogdell, R. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Organic and Biomolecular Chemistry
Journal Abbr.:Org. Biomol. Chem.
Publisher:Royal Society of Chemistry
ISSN:1477-0520
ISSN (Online):1477-0539
Copyright Holders:Copyright © 2016 Royal Society of Chemistry
First Published:First published in Organic and Biomolecular Chemistry 14(4):1359-1362
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
561421Plasmon Enhanced Pyroelectric Nanoscale Trapping and SensingAlasdair ClarkThe Royal Academy of Engineering (RAE)10216/103ENG - BIOMEDICAL ENGINEERING
690421Glasgow Molecular Pathology (GMP) NodeKarin OienMedical Research Council (MRC)MR/N005813/1ICS - EXPERIMENTAL THERAPEUTICS