Fluorous-directed assembly of DNA origami nanostructures

Zou, J. et al. (2023) Fluorous-directed assembly of DNA origami nanostructures. ACS Nano, 17(1), pp. 752-759. (doi: 10.1021/acsnano.2c10727) (PMID:36537902) (PMCID:PMC9835977)

Abstract

An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson–Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated “catch-and-latch” approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Santana Vega, Dr Marina and Peveler, Dr William and Clark, Professor Alasdair and Withers, Dr Jamie and Christie, Mr Iain and Stammers, Dr Ashley and Zou, Jiajia and Aekbote, Dr Badri and Burley, Dr Glenn
• Authors: Zou, J., Stammers, A. C., Taladriz-Sender, A., Withers, J. M., Christie, I., Santana Vega, M., Aekbote, B. L., Peveler, W. J., Rusling, D. A., Burley, G. A., and Clark, A. W.
• College/School: College of Science and Engineering > School of Chemistry; College of Science and Engineering > School of Engineering; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Nano
• Publisher: American Chemical Society
• ISSN: 1936-0851
• ISSN (Online): 1936-086X
• Published Online: 20 December 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in ACS Nano 17(1): 752-759
• Publisher Policy: Reproduced under a Creative Commons License
• Data DOI: 10.5525/gla.researchdata.1353

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
301408		Macromolecular construction of nucleic acid networks directed by the flourous effect: A new paradigm for the programmable assembly of molecular information	Alasdair Clark	Leverhulme Trust (LEVERHUL)	RPG-2018-149/ 170977	ENG - Biomedical Engineering
172826		DNA-directed construction of three-dimensional photosynthetic assemblies	Alasdair Clark	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/N016734/1	ENG - Biomedical Engineering
309846		Decentralised water technologies	William Sloan	Engineering and Physical Sciences Research Council (EPSRC)	EP/V030515/1	ENG - Infrastructure & Environment