Nucleotide sequence Of the feline Bmi-1 coding region

Kinnon, S. and Fulton, R. (1999) Nucleotide sequence Of the feline Bmi-1 coding region. DNA Sequence: The Journal of DNA Sequencing and Mapping, 10(4-5), pp. 335-338. (doi:10.3109/10425179909033960)

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Publisher's URL: http://dx.doi.org/10.3109/10425179909033960

Abstract

The bmi-1 gene was discovered as a clonal integration site of the Moloney murine leukaemia virus in B-cell lymphomas. The Bmi-1 protein contains the RING finger motif and is homologous to two Drosophila proteins known to be part of a multimeric protein complex involved in repressing gene transcription. A similar role for the highly conserved Bmi-1 protein in mammalian cells has been suggested. The coding regions for the mouse and human genes are known and are 92% homologous. This study involved PCR amplification, cloning, and sequencing of the 980bp feline bmi-1 coding region which was shown to be 92% and 97% homologous to the mouse and human genes respectively. From the open reading frame the feline protein is 326 amino acids in length and is 99% homologous to the human protein and 97% homologous to the mouse protein. This data is consistent with the closer relationship between the feline and human genomes and provides another experimental system in which to analyse Bmi-1 function.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fulton, Dr Ruth
Authors: Kinnon, S., and Fulton, R.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:DNA Sequence: The Journal of DNA Sequencing and Mapping
Publisher:Taylor and Francis
ISSN:1042-5179
ISSN (Online):1029-2365

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