Impact of HDL on adipose tissue metabolism and adiponectin expression

Van Linthout, S. et al. (2010) Impact of HDL on adipose tissue metabolism and adiponectin expression. Atherosclerosis, 210(2), pp. 438-444. (doi: 10.1016/j.atherosclerosis.2010.01.001)

Abstract

<b>Objective</b> The objective of the current study was to investigate the hypothesis that high-density lipoprotein (HDL) influences adipocyte metabolism and adiponectin expression. Therefore, HDL was increased in vivo via apolipoprotein (apo) A-I gene transfer and in vitro via supplementation of HDL to partly differentiated adipocytes, in the presence or absence of lipopolysaccharide (LPS), known to decrease HDL cholesterol and adiponectin levels in vivo.<p></p> <b>Methods and results</b> Apo A-I transfer resulted in a significant increase of HDL cholesterol in control and LPS-injected C57BL/6 mice, which was paralleled by an increase in plasma adiponectin levels and adiponectin expression in abdominal fat. Triglyceride and free fatty acids levels after LPS administration were 2.2-fold (p < 0.05) and 1.3-fold (p < 0.05) lower, respectively, in Ad.hapoA-I-LPS than in Ad.Null-LPS mice. In parallel, the LPS-induced mRNA expression of hormone sensitive lipase was 3.5-fold (p = 0.05) decreased in the Ad.hapoA-I-LPS group. On the other hand, apo A-I transfer abrogated the LPS-mediated reduction in lipin-1 and CD36 mRNA expression by 8.2-fold (p < 0.05) and 18-fold (p < 0.05), respectively. Concomitantly, the phosphorylation state of Akt was 2.0-fold (p < 0.05) increased in the Ad.hapoA-I-LPS compared to theAd.Null-LPS group. Pre-incubation of partly differentiated adipocytes with HDL (50 μg protein/ml) increased adiponectin expression by 1.5-fold under basal conditions (p < 0.05) and could abrogate LPS-induced down-regulation of adiponectin, both in a phosphatidylinositol-3-kinase-dependent manner.<p></p> <b>Conclusions</b> HDL affects adipocyte metabolism and adiponectin expression.<p></p>

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Meloni, Dr Marco
• Authors: Van Linthout, S., Foryst-Ludwig, A., Spillmann, F., Peng, J., Feng, Y., Meloni, M., Van Craeyveld, E., Kintscher, U., Schultheiss, H.-P., De Geest, B., and Tschöpe, C.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Atherosclerosis
• Publisher: Elsevier Ireland Ltd
• ISSN: 0021-9150
• ISSN (Online): 1879-1484