Salmonella enterica Serovar TyphimuriumsurA mutants are attenuated and effective live oral vaccines

Sydenham, M., Douce, G. , Bowe, F., Ahmed, S., Chatfield, S. and Dougan, G. (2000) Salmonella enterica Serovar TyphimuriumsurA mutants are attenuated and effective live oral vaccines. Infection and Immunity, 68(3), pp. 1109-1115. (doi: 10.1128/IAI.68.3.1109-1115.2000)

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A previously described attenuated TnphoA mutant (BRD441) of Salmonella enterica serovar Typhimurium C5 (I. Miller, D. Maskell, C. Hormaeche, K. Johnson, D. Pickard, and G. Dougan, Infect. Immun. 57:2758–2763, 1989) was characterized, and the transposon was shown to be inserted in surA, a gene which encodes a peptidylprolyl-cis,trans-isomerase. A defined surA deletion mutation was introduced into S. enterica serovar Typhimurium C5 and the mutant strain, namedS. enterica serovar Typhimurium BRD1115, was extensively characterized both in vitro and in vivo. S. entericaserovar Typhimurium BRD1115 was found to be defective in the ability to adhere to and invade eukaryotic cells. Furthermore, S. enterica serovar Typhimurium BRD1115 was attenuated by at least 3 log units when administered orally or intravenously to BALB/c mice. Complementation of the mutation with a plasmid carrying the intactsurA gene almost completely restored the virulence of BRD1115. In addition, S. enterica serovar Typhimurium BRD1115 demonstrated potential as a vaccine candidate, since mice immunized with BRD1115 were protected against subsequent challenge withS. enterica serovar Typhimurium C5. S. entericaserovar Typhimurium BRD1115 also showed potential as a vehicle for the effective delivery of heterologous antigens, such as the nontoxic, protective fragment C domain of tetanus toxin, to the murine immune system.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Douce, Dr Gillian
Authors: Sydenham, M., Douce, G., Bowe, F., Ahmed, S., Chatfield, S., and Dougan, G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Infection and Immunity
Publisher:American Society for Microbiology
ISSN (Online):1098-5522

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