Rebourcet, D., O'Shaughnessy, P. J. , Monteiro, A., Milne, L., Cruickshanks, L., Jeffrey, N., Guillou, F., Freeman, T. C., Mitchell, R. T. and Smith, L. B. (2014) Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis. PLoS ONE, 9(8), e105687. (doi: 10.1371/journal.pone.0105687) (PMID:25144714) (PMCID:PMC4140823)
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Publisher's URL: http://dx.doi.org/10.1371/journal.pone.0105687
Abstract
The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transgenic mouse model in which Amh-Cre induces expression of the receptor for Diphtheria toxin (iDTR) specifically within Sertoli cells. This causes controlled, cell-specific and acute ablation of the Sertoli cell population in the adult animal following Diphtheria toxin injection. Results show that Sertoli cell ablation leads to rapid loss of all germ cell populations. In addition, adult Leydig cell numbers decline by 75% with the remaining cells concentrated around the rete and in the sub-capsular region. In the absence of Sertoli cells, peritubular myoid cell activity is reduced but the cells retain an ability to exclude immune cells from the seminiferous tubules. These data demonstrate that, in addition to support of spermatogenesis, Sertoli cells are required in the adult testis both for retention of the normal adult Leydig cell population and for support of normal peritubular myoid cell function. This has implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Monteiro, Dr Ana and O'Shaughnessy, Professor Peter |
Authors: | Rebourcet, D., O'Shaughnessy, P. J., Monteiro, A., Milne, L., Cruickshanks, L., Jeffrey, N., Guillou, F., Freeman, T. C., Mitchell, R. T., and Smith, L. B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
Copyright Holders: | Copyright © 2014 The Authors |
First Published: | First published in PLoS One 9(8):e105687 |
Publisher Policy: | Reproduced under a Creative Commons License |
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