Yang, J., McNamara, L. E., Gadegaard, N. , Alakpa, E. V., Burgess, K. V., Meek, D. and Dalby, M. J. (2014) Nanotopographical induction of osteogenesis through adhesion, bone morphogenic protein co-signaling and regulation of microRNAs. ACS Nano, 8(10), pp. 9941-9953. (doi: 10.1021/nn504767g) (PMID:25227207)
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Abstract
It is emerging that nanotopographical information can be used to induce osteogenesis from mesenchymal stromal cells from the bone marrow and it is hoped that this nanoscale bioactivity can be utilized to engineer next generation implants. However, the osteogenic mechanism of surfaces is currently poorly understood. In this report, we investigate mechanism and implicate bone morphogenic protein (BMP) in up-regulation of RUNX2 and show that RUNX2 and its regulatory miRNAs are BMP sensitive. Our data demonstrates that osteogenic nanotopography promotes co-localization of intergrins and BMP2 receptors in order to enhance osteogenic activity and that vitronectin is important in this interface. This provides insight that topographical regulation of adhesion can have effects on signaling cascades outside of cytoskeletal signaling and that adhesions can have roles in augmenting BMP signaling.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Dalby, Professor Matthew and Alakpa, Miss Enateri and Burgess, Dr Karl and McNamara, Dr Laura and Meek, Professor Dominic and Gadegaard, Professor Nikolaj and Yang, Dr Jingli |
| Authors: | Yang, J., McNamara, L. E., Gadegaard, N., Alakpa, E. V., Burgess, K. V., Meek, D., and Dalby, M. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Geographical and Earth Sciences |
| Journal Name: | ACS Nano |
| Publisher: | American Chemical Society |
| ISSN: | 1936-0851 |
| ISSN (Online): | 1936-086X |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in ACS Nano 8(10):9941-9953 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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