Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii

Jimenez-Ruiz, E. , Wong, E. H., Pall, G. S. and Meissner, M. (2014) Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii. Parasitology, 141(11), pp. 1390-1398. 24926834. (doi: 10.1017/S0031182014000559)

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The dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of the pitfalls involved in gene-function analysis using these systems that can lead to wrong or over-interpretation of phenotypes. We will also mention different options to standardize the application of these techniques for the characterization of gene function in high-throughput.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Jimenez Ruiz, Dr Elena and Meissner, Professor Markus and Wong, Dr Eleanor and Pall, Dr Gurman
Authors: Jimenez-Ruiz, E., Wong, E. H., Pall, G. S., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Parasitology
Publisher:Cambridge University Press
ISSN (Online):1469-8161
Copyright Holders:Copyright © 2014 Cambridge University Press
First Published:First published in Parasitology 141(11):1390-1398
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501601Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY
371793The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)073990/Z/03/BIII - PARASITOLOGY