Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes

Yuan, X. , Chim, Y. and Yin, H. (2014) Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes. In: Optical Elastography and Tissue Biomechanics, San Francisco, CA, USA, 1-2 Feb 2014, 89460M. (doi: 10.1117/12.2039859)

Abstract

Autologous chondrocyte transplantation (ACT) has become a promising method for repairing large articular defects. However, dedifferentiation of chondrocytes during cell expansion remains a major limitation for ACT procedures. In this study, we explore the potential of confining cell shape for re-differentiation of dedifferentiated bovine chondrocytes. A novel culture system, combining 2D micropatterning with 3D matrix formation, was developed to control and maintain individual chondrocyte’s shape. Both collagen II synthesis and the mechanical properties of cells were monitored during re-differentiation. We show that a spherical morphology without cell spreading plays a limited role in induction of re-differentiation. Instead, isolated, dedifferentiated chondrocytes partially regain chondrogenic properties if they have an appropriate cell shape and limited spreading.

• Item Type: Conference Proceedings
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Yin, Professor Huabing and Yuan, Dr Xiaofei
• Authors: Yuan, X., Chim, Y., and Yin, H.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• ISSN: 0277786X