Fogg, P. C.M., Colloms, S. , Rosser, S., Stark, M. and Smith, M. C.M. (2014) New applications for phage integrases. Journal of Molecular Biology, 426(15), pp. 2703-2716. (doi: 10.1016/j.jmb.2014.05.014) (PMID:24857859) (PMCID:PMC4111918)
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Abstract
Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Rosser, Professor Susan and Stark, Professor Marshall and Colloms, Dr Sean |
| Authors: | Fogg, P. C.M., Colloms, S., Rosser, S., Stark, M., and Smith, M. C.M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Journal of Molecular Biology |
| Publisher: | Elsevier Ltd. |
| ISSN: | 0022-2836 |
| ISSN (Online): | 1089-8638 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Journal of Molecular Biology 426(15):2703-2716 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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