A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants

Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B., Clements, G.B. and Thomas, D.G.T. (1987) A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants. Journal of Neuro-Oncology, 4(4), pp. 389-396. (doi: 10.1007/BF00195610)

Abstract

Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV specified proteins, and one monoclonal antibody TI81 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. TI81 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the TI81-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV I infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kennedy, Professor Peter
• Authors: Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B., Clements, G.B., and Thomas, D.G.T.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Neuro-Oncology
• Publisher: Springer Verlag
• ISSN: 0167-594X
• ISSN (Online): 1573-7373