Demonstration of JC virus by immunofluorescence in multiple cell types in experimentally infected adult human brain cell cultures

Wroblewska, Z., Kennedy, P. G.E., Wellish, M. C., Lisak, R. P. and Gilden, D. H. (1982) Demonstration of JC virus by immunofluorescence in multiple cell types in experimentally infected adult human brain cell cultures. Journal of the Neurological Sciences, 54(2), pp. 189-196. (doi: 10.1016/0022-510X(82)90181-2)

Abstract

JC virus (JCV)-induced T antigen developed in the nuclei of approximately 50% of adult human brain (AHB) cells infected with JCV. Approximately 10% of these T antigen-positive cells contained glial fibrillary acidic protein (GFAP) and were identified as astrocytes in immunofluorescence studies. About 50% of these T antigen-positive cells bound antiserum to fibronectin on their surface and were identified as fibroblasts. No cells binding antiserum to galactocerebroside (GC), an oligodendrocyte-specific marker, were detected in infected or non-infected AHB cultures. The cell type of AHB that constitutes the remaining 40–50% of T antigen-positive cells that are GFAP-, fibronectin- and GC-negative remains to be determined.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kennedy, Professor Peter
• Authors: Wroblewska, Z., Kennedy, P. G.E., Wellish, M. C., Lisak, R. P., and Gilden, D. H.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of the Neurological Sciences
• Publisher: Elsevier
• ISSN: 0022-510X
• ISSN (Online): 1878-5883