Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation

Rodrigo-Navarro, A. , Rico, P., Saadeddin, A., Garcia, A. J. and Salmeron-Sanchez, M. (2014) Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation. Scientific Reports, 4, 5849. (doi: 10.1038/srep05849) (PMID:25068919) (PMCID:PMC5376178)

Abstract

Genetically modified Lactococcus lactis, non-pathogenic bacteria expressing the FNIII7-10 fibronectin fragment as a protein membrane have been used to create a living biointerface between synthetic materials and mammalian cells. This FNIII7-10 fragment comprises the RGD and PHSRN sequences of fibronectin to bind α5β1 integrins and triggers signalling for cell adhesion, spreading and differentiation. We used L. lactis strain to colonize material surfaces and produce stable biofilms presenting the FNIII7-10 fragment readily available to cells. Biofilm density is easily tunable and remains stable for several days. Murine C2C12 myoblasts seeded over mature biofilms undergo bipolar alignment and form differentiated myotubes, a process triggered by the FNIII7-10 fragment. This biointerface based on living bacteria can be further modified to express any desired biochemical signal, establishing a new paradigm in biomaterial surface functionalisation for biomedical applications.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Rodrigo-Navarro, Mr Aleixandre
• Authors: Rodrigo-Navarro, A., Rico, P., Saadeddin, A., Garcia, A. J., and Salmeron-Sanchez, M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Scientific Reports
• Publisher: Nature Publishing Group
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2014 The Authors
• First Published: First published in Scientific Reports 4(5849)
• Publisher Policy: Reproduced under a Creative Commons License