Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Hay, R. F., Gibson, G. M. , Lee, M. P., Padgett, M. J. and Phillips, D. B. (2014) Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation. Optics Express, 22(15), pp. 18662-18667. (doi: 10.1364/OE.22.018662)

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High-speed video stereo-microscopy relies on illumination from two distinct angles to create two views of a sample from different directions. The 3D trajectory of a microscopic object can then be reconstructed using parallax to combine 2D measurements of its position in each image. In this work, we evaluate the accuracy of 3D particle tracking using this technique, by extending the number of views from two to four directions. This allows us to record two independent sets of measurements of the 3D coordinates of tracked objects, and comparison of these enables measurement and minimisation of the tracking error in all dimensions. We demonstrate the method by tracking the motion of an optically trapped microsphere of 5 μm in diameter, and find an accuracy of 2–5 nm laterally, and 5–10 nm axially, representing a relative error of less than 2.5% of its range of motion in each dimension.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Gibson, Dr Graham and Padgett, Professor Miles and Phillips, Dr David
Authors: Hay, R. F., Gibson, G. M., Lee, M. P., Padgett, M. J., and Phillips, D. B.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
Copyright Holders:Copyright © 2014 Optical Society of America
First Published:First published in Optics Express 22(15):18662-18667
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
542471Optical Atomic Force MicroscopyMiles PadgettEngineering & Physical Sciences Research Council (EPSRC)EP/I007822/1P&A - PHYSICS & ASTRONOMY