Ricard, C., Coles, J. A., Serduc, R., van der Sanden, B., Verant, P. and Vial, J.-C. (2009) Two-photon imaging. In: Squire, L. R. (ed.) Encyclopedia of Neuroscience. Elsevier: Amsterdam, pp. 1221-1229. ISBN 9780080450469 (doi: 10.1016/B978-008045046-9.00863-9)
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Publisher's URL: http://dx.doi.org/10.1016/B978-008045046-9.00863-9
Abstract
A two-photon microscope is basically a scanning confocal microscope that uses for excitation near-infrared light delivered in intense, femtosecond pulses. It allows imaging with the spatial resolution of a conventional optical microscope (<1 μm) but can perform such imaging through several hundred micrometers of brain tissue. In this article, the physical principles of two-photon imaging are explained, and imaging in the cortex of the living mouse of neurons, glia, and microvasculature is described. Finally, the advantages of two-photon microscopy for studying neuropathologies including tumors are summarized.
Item Type: | Book Sections |
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Status: | Published |
Glasgow Author(s) Enlighten ID: | Coles, Dr Jonathan |
Authors: | Ricard, C., Coles, J. A., Serduc, R., van der Sanden, B., Verant, P., and Vial, J.-C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Publisher: | Elsevier |
ISBN: | 9780080450469 |
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