Interactions among mitochondrial proteins altered in glioblastoma

Deighton, R. F., Le Bihan, T., Martin, S. F., Gerth, A. M. J., McCulloch, M., Edgar, J. M. , Kerr, L. E., Whittle, I. R. and McCulloch, J. (2014) Interactions among mitochondrial proteins altered in glioblastoma. Journal of Neuro-Oncology, 118(2), pp. 247-256. (doi: 10.1007/s11060-014-1430-5)

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Abstract

Mitochondrial dysfunction is putatively central to glioblastoma (GBM) pathophysiology but there has been no systematic analysis in GBM of the proteins which are integral to mitochondrial function. Alterations in proteins in mitochondrial enriched fractions from patients with GBM were defined with label-free liquid chromatography mass spectrometry. 256 mitochondrially-associated proteins were identified in mitochondrial enriched fractions and 117 of these mitochondrial proteins were markedly (fold-change ≥2) and significantly altered in GBM (p ≤ 0.05). Proteins associated with oxidative damage (including catalase, superoxide dismutase 2, peroxiredoxin 1 and peroxiredoxin 4) were increased in GBM. Protein–protein interaction analysis highlighted a reduction in multiple proteins coupled to energy metabolism (in particular respiratory chain proteins, including 23 complex-I proteins). Qualitative ultrastructural analysis in GBM with electron microscopy showed a notably higher prevalence of mitochondria with cristolysis in GBM. This study highlights the complex mitochondrial proteomic adjustments which occur in GBM pathophysiology.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McCulloch, Mr James and Edgar, Professor Julia
Authors: Deighton, R. F., Le Bihan, T., Martin, S. F., Gerth, A. M. J., McCulloch, M., Edgar, J. M., Kerr, L. E., Whittle, I. R., and McCulloch, J.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Neuro-Oncology
Publisher:Springer US
ISSN:0167-594X
ISSN (Online):1573-7373
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Neuro-Oncology 118(2):247-256
Publisher Policy:Reproduced under a Creative Commons License

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