Paraoxonase 2 protein is spatially expressed in the human placenta and selectively reduced in labour

Alwarfaly, S., ABDULSID, A., Hanretty, K. and Lyall, F. (2014) Paraoxonase 2 protein is spatially expressed in the human placenta and selectively reduced in labour. PLoS ONE, 9(5), e96754. (doi: 10.1371/journal.pone.0096754) (PMID:24816800) (PMCID:PMC4016019)

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Abstract

Humans parturition involves interaction of hormonal, neurological, mechanical stretch and inflammatory pathways and the placenta plays a crucial role. The paraoxonases (PONs 1–3) protect against oxidative damage and lipid peroxidation, modulation of endoplasmic reticulum stress and regulation of apoptosis. Nothing is known about the role of PON2 in the placenta and labour. Since PON2 plays a role in oxidative stress and inflammation, both features of labour, we hypothesised that placental PON2 expression would alter during labour. PON2 was examined in placentas obtained from women who delivered by cesarean section and were not in labour and compared to the equivalent zone of placentas obtained from women who delivered vaginally following an uncomplicated labour. Samples were obtained from 12 sites within each placenta: 4 equally spaced apart pieces were sampled from the inner, middle and outer placental regions. PON2 expression was investigated by Western blotting and real time PCR. Two PON2 forms, one at 62 kDa and one at 43 kDa were found in all samples. No difference in protein expression of either isoform was found between the three sites in either the labour or non-labour group. At the middle site there was a highly significant decrease in PON2 expression in the labour group when compared to the non-labour group for both the 62 kDa form (p = 0.02) and the 43 kDa form (p = 0.006). No spatial differences were found within placentas at the mRNA level in either labour or non-labour. There was, paradoxically, an increase in PON2 mRNA in the labour group at the middle site only. This is the first report to describe changes in PON2 in the placenta in labour. The physiological and pathological significance of these remains to be elucidated but since PON2 is anti-inflammatory further studies are warranted to understand its role.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:ABDULSID, Akrem and Hanretty, Dr Kevin and Lyall, Professor Fiona
Authors: Alwarfaly, S., ABDULSID, A., Hanretty, K., and Lyall, F.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS One 9(5):e96754
Publisher Policy:Reproduced under a Creative Commons License

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