iRFP is a real time marker for transformation based assays in high content screening

Paulus-Hock, V., Cheung, E. C., Roxburgh, P. , Vousden, K. H. and Hock, A. K. (2014) iRFP is a real time marker for transformation based assays in high content screening. PLoS ONE, 9(6), e98399. (doi: 10.1371/journal.pone.0098399) (PMID:24887316) (PMCID:PMC4041769)

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Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with high throughput systems, in contrast to traditional methods which are often labor-intensive, subjective to bias and do not allow further analysis using the same cells. Transformation in a single layer soft agar assay could be documented as early as 2 to 3 days in a 96 well format, which can be easily combined with standard transfection, infection and compound screening setups to allow for high throughput screening to identify therapeutic targets.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cheung, Mr Eric and Hock, Dr Andreas and Vousden, Karen and Paulus-Hock, Mrs Viola and Roxburgh, Dr Patricia
Authors: Paulus-Hock, V., Cheung, E. C., Roxburgh, P., Vousden, K. H., and Hock, A. K.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS One 9(6):e98399
Publisher Policy:Reproduced under a Creative Commons License

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