Investigation of the limits of nanoscale filopodial interactions

McNamara, L. E., Sjostrom, T., Seunarine, K., Meek, R.M. D., Su, B. and Dalby, M. J. (2014) Investigation of the limits of nanoscale filopodial interactions. Journal of Tissue Engineering, 5, p. 2041731414536177. (doi: 10.1177/2041731414536177) (PMID:24904726) (PMCID:PMC4046805)

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Mesenchymal stem cells are sensitive to changes in feature height, order and spacing. We had previously noted that there was an inverse relationship between osteoinductive potential and feature height on 15-, 55- and 90 nm-high titania nanopillars, with 15 nm-high pillars being the most effective substrate at inducing osteogenesis of human mesenchymal stem cells. The osteoinductive effect was somewhat diminished by decreasing the feature height to 8 nm, however, which suggested that there was a cut-off point, potentially associated with a change in cell–nanofeature interactions. To investigate this further, in this study, a scanning electron microscopy/three-dimensional scanning electron microscopy approach was used to examine the interactions between mesenchymal stem cells and the 8 and 15 nm nanopillared surfaces. As expected, the cells adopted a predominantly filopodial mode of interaction with the 15 nm-high pillars. Interestingly, fine nanoscale membrane projections, which we have termed ‘nanopodia,’ were also employed by the cells on the 8 nm pillars, and it seems that this is analogous to the cells ‘clinging on with their fingertips’ to this scale of features.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Seunarine, Dr Krishna and Dalby, Professor Matthew and McNamara, Dr Laura and Meek, Professor Dominic
Authors: McNamara, L. E., Sjostrom, T., Seunarine, K., Meek, R.M. D., Su, B., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering
Publisher:SAGE Publications
ISSN (Online):2041-7314
Published Online:15 May 2014
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Tissue Engineering 5: 2041731414536177
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502891Micro- and nano-patterning of titanium surfaces for optimal osseointegration of orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/G048703/1RI MOLECULAR CELL & SYSTEMS BIOLOGY