Daneshvar, H., Namazi, M. J., Kamiabi, H., Burchmore, R. , Cleaveland, S. and Phillips, S. (2014) Gentamicin-attenuated leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran. PLoS Neglected Tropical Diseases, 8(4), e2757. (doi: 10.1371/journal.pntd.0002757) (PMID:24743691) (PMCID:PMC3990512)
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Abstract
An attenuated line of Leishmania infantum (L. infantum H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A vaccine trial was conducted using 103 naive dogs from a leishmaniosis non-endemic area (55 vaccinated and 48 unvaccinated) brought into an endemic area of southeast Iran. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination. The efficacy of the vaccine was evaluated after 24 months (4 sandfly transmission seasons) by serological, parasitological analyses and clinical examination. In western blot analysis of antibodies to L. infantum antigens, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs which were seropositive at >100, recognized the 21 kDa antigen of L. infantum wild-type (WT). Nine out of 31 (29%) unvaccinated dogs, but none of vaccinated dogs, were positive for the presence of Leishmania DNA. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. These results suggest that gentamicin-attenuated L. infantum induced a significant and strong protective effect against canine visceral leishmaniosis in the endemic area.
Item Type: | Articles |
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Additional Information: | A correction to this article is available at https://doi.org/10.1371/journal.pntd.0003787. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Namazi, Dr Mohammad and Daneshvar, Dr Hamid and Phillips, Professor Stephen and Burchmore, Dr Richard and Cleaveland, Professor Sarah |
Authors: | Daneshvar, H., Namazi, M. J., Kamiabi, H., Burchmore, R., Cleaveland, S., and Phillips, S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | PLoS Neglected Tropical Diseases |
Publisher: | Public Library of Science |
ISSN: | 1935-2727 |
ISSN (Online): | 1935-2735 |
Copyright Holders: | Copyright © 2014 The Authors |
First Published: | First published in PLoS Neglected Tropical Diseases 8(4):e2757 |
Publisher Policy: | Reproduced under a Creative Commons License |
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