Roszak, A.W., Gardiner, A.T., Isaacs, N.W. and Cogdell, R.J. (2007) Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides. Biochemistry, 46(11), pp. 2909-2916. (doi: 10.1021/bi062154i)
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Abstract
This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cogdell, Professor Richard and Isaacs, Professor Neil and Roszak, Dr Aleksander |
| Authors: | Roszak, A.W., Gardiner, A.T., Isaacs, N.W., and Cogdell, R.J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Chemistry |
| Journal Name: | Biochemistry |
| ISSN: | 0006-2979 |
| ISSN (Online): | 1608-3040 |
| Published Online: | 23 February 2007 |
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