Bourquin, Y., Syed, A., Reboud, J. , Ranford-Cartwright, L. C. , Barrett, M. P. and Cooper, J. M. (2014) Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics. Angewandte Chemie (International Edition), 53(22), pp. 5587-5590. (doi: 10.1002/anie.201310401) (PMID:24677583) (PMCID:PMC4232275)
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Abstract
One significant challenge in medical diagnostics lies in the development of label-free methods to separate different cells within complex biological samples. Here we demonstrate a generic, low-power ultrasonic separation technique, able to enrich different cell types based upon their physical properties. For malaria, we differentiate between infected and non-infected red blood cells in a fingerprick-sized drop of blood. We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005 %). In a second example, we also show that our methods can be used to enrich different cell types, concentrating Trypanosoma in blood at very low levels of infection, on disposable, low-cost chips.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ranford-Cartwright, Dr Lisa and Cooper, Professor Jonathan and Reboud, Dr Julien and Syed, Miss Abeer and Barrett, Professor Michael |
| Authors: | Bourquin, Y., Syed, A., Reboud, J., Ranford-Cartwright, L. C., Barrett, M. P., and Cooper, J. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Angewandte Chemie (International Edition) |
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
| ISSN: | 1433-7851 |
| ISSN (Online): | 1521-3773 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Angewandte Chemie 53(22):5587–5590 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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