Wiejak, J., Dunlop, J. and Yarwood, S. J. (2014) The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs. FEBS Letters, 588(9), pp. 1556-1561. (doi: 10.1016/j.febslet.2014.02.038)
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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2014.02.038
Abstract
The cyclic AMP sensor, EPAC1, activates AP1-mediated transcription in HUVECs. Correspondingly, induction of the SOCS3 minimal promoter by EPAC1 requires a single AP1 site that constitutively binds phosphorylated (Ser63) c-Jun in DNA-pull-down assays. c-Jun (Ser63) becomes further phosphorylated following cyclic AMP stimulation and specific activation of protein kinase A (PKA), but not through selective activation of EPAC1. Moreover, despite a requirement for c-Jun for SOCS3 induction in fibroblasts, phospho-null c-Jun (Ser63/73Ala) had little effect on SOCS3 induction by cyclic AMP in HUVECs. AP1 activation and SOCS3 induction by EPAC1 in HUVECs therefore occur independently of c-Jun phosphorylation on Ser63.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yarwood, Dr Stephen and Dunlop, Mrs Julia and Wiejak, Dr Jolanta |
| Authors: | Wiejak, J., Dunlop, J., and Yarwood, S. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | FEBS Letters |
| Publisher: | Elsevier |
| ISSN: | 0014-5793 |
| ISSN (Online): | 1873-3468 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Febs Letters 588(9):1556-1561 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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