The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs

Wiejak, J., Dunlop, J. and Yarwood, S. J. (2014) The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs. FEBS Letters, 588(9), pp. 1556-1561. (doi: 10.1016/j.febslet.2014.02.038)

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The cyclic AMP sensor, EPAC1, activates AP1-mediated transcription in HUVECs. Correspondingly, induction of the SOCS3 minimal promoter by EPAC1 requires a single AP1 site that constitutively binds phosphorylated (Ser63) c-Jun in DNA-pull-down assays. c-Jun (Ser63) becomes further phosphorylated following cyclic AMP stimulation and specific activation of protein kinase A (PKA), but not through selective activation of EPAC1. Moreover, despite a requirement for c-Jun for SOCS3 induction in fibroblasts, phospho-null c-Jun (Ser63/73Ala) had little effect on SOCS3 induction by cyclic AMP in HUVECs. AP1 activation and SOCS3 induction by EPAC1 in HUVECs therefore occur independently of c-Jun phosphorylation on Ser63.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Yarwood, Dr Stephen and Dunlop, Mrs Julia and Wiejak, Dr Jolanta
Authors: Wiejak, J., Dunlop, J., and Yarwood, S. J.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:FEBS Letters
ISSN (Online):1873-3468
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Febs Letters 588(9):1556-1561
Publisher Policy:Reproduced under a Creative Commons License

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