Smith, D.G.E., Russell, W.C. and Thirkell, D. (1994) Adherence of Ureaplasma urealyticum to human epithelial cells. Microbiology, 140(10), pp. 2893-2898. (doi: 10.1099/00221287-140-10-2893)
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Publisher's URL: http://dx.doi.org/10.1099/00221287-140-10-2893
Abstract
Adherence of <i>Ureaplasma urealyticum</i> cells to eukaryotic cell monolayers was quantified using the Bertholet assay to monitor ammonia produced from urea by ureaplasma urease. Adherence was abolished by pre-treatment of ureaplasmas with HeLa cell extracts and inhibited to varying degrees by pre-treatment of the ureaplasmas with N-acetylneuraminic acid, specific antisera and monoclonal antibodies. The data suggest the presence of several ureaplasma adhesins, some of which are species- or serotype-specific and some of which are proteinaceous and antigenic. The serotype-8-specific 96 kDa surface-expressed antigen may be one adhesin. Pre-treatment of HeLa cell monolayers with neuraminidase significantly reduced ureaplasma adherence and, using a novel ‘immunoblot adherence assay’, ureaplasmas were shown to bind to a number of HeLa cell components, three of which appear to terminate in sialic acid.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smith, Professor David |
| Authors: | Smith, D.G.E., Russell, W.C., and Thirkell, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Microbiology |
| Publisher: | Society for General Microbiology |
| ISSN: | 1350-0872 |
| ISSN (Online): | 1465-2080 |
| Related URLs: |
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