Dynamics of cyclic-AMP efflux in relation to α-MSH secretion from melanotrope cells of Xenopus laevis

De Koning, H. P. , Jenks, B. G., Huchede, B. and Roubos, E. W. (1992) Dynamics of cyclic-AMP efflux in relation to α-MSH secretion from melanotrope cells of Xenopus laevis. Life Sciences, 51(21), pp. 1667-1673. (doi: 10.1016/0024-3205(92)90311-C)

Abstract

An important factor in regulating secretion from endocrine cells is the cytoplasmic concentration of cyclic-AMP. Many regulatory substances are known to either stimulate or inhibit the production of this second messenger through activation of their receptors. In the present study, we have monitored changes in cyclic-AMP efflux from melanotrope cells of <i>Xenopus laevis</i> in response to established neurochemical regulators of α-MSH secretion. In vitro superfusion of neurointermediate lobes allows for a dynamic recording of cyclic-AMP production in relation to hormone secretion. Unlike α-MSH secretion, the efflux of cyclic-AMP was not dependent on the concentration of extracellular calcium, indicating that hormone release and cyclic-AMP efflux are mediated by different mechanisms. The phosphodiesterase inhibitor IBMX and the adenylate cyclase activator forskolin stimulated cyclic-AMP efflux, but had no stimulatory effect on α-MSH release. This indicates that an increase in cyclic-AMP production in melanotrope cells is not necessarily accompanied by an increase in the rate of α-MSH release. Corticotropin-releasing factor stimulated cyclic-AMP efflux with dynamics similar to that induced by the amphibian peptide sauvagine. Dopamine and the GABAB receptor agonist baclofen both inhibited cyclic-AMP efflux and α-MSH release, with similar dynamics of inhibition and similar dose-response relationships. It is proposed that an inhibition of cyclic-AMP efflux is coupled to an inhibition of α-MSH secretion.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: De Koning, Professor Harry
• Authors: De Koning, H. P., Jenks, B. G., Huchede, B., and Roubos, E. W.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Life Sciences
• Publisher: Elsevier
• ISSN: 0024-3205
• ISSN (Online): 1879-0631