Kremer, C., Witte, C., Neale, S. L. , Reboud, J. , Barrett, M. P. and Cooper, J. M. (2014) Shape-dependent optoelectronic cell lysis. Angewandte Chemie (International Edition), 53(3), pp. 842-846. (doi: 10.1002/anie.201307751) (PMID:24402800) (PMCID:PMC4441254)
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Abstract
We show an electrical method to break open living cells amongst a population of different cell types, where cell selection is based upon their shape. We implement the technique on an optoelectronic platform, where light, focused onto a semiconductor surface from a video projector creates a reconfigurable pattern of electrodes. One can choose the area of cells to be lysed in real-time, from single cells to large areas, simply by redrawing the projected pattern. We show that the method, based on the “electrical shadow” that the cell casts, allows the detection of rare cell types in blood (including sleeping sickness parasites), and has the potential to enable single cell studies for advanced molecular diagnostics, as well as wider applications in analytical chemistry.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Neale, Professor Steven and Witte, Dr Christian and Cooper, Professor Jonathan and Reboud, Professor Julien and Barrett, Professor Michael |
| Authors: | Kremer, C., Witte, C., Neale, S. L., Reboud, J., Barrett, M. P., and Cooper, J. M. |
| Subjects: | Q Science > Q Science (General) |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Angewandte Chemie (International Edition) |
| Journal Abbr.: | Angew Chem Int Ed Engl |
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
| ISSN: | 1433-7851 |
| ISSN (Online): | 1521-3773 |
| Copyright Holders: | Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
| First Published: | First published in Angewandte Chemie (International Edition) 53(3):842-846 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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