Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Jeorrett, A., Neale, S.L. , Massoubre, D., Gu, E., Henderson, R.K., Millington, O., Mathieson, K. and Dawson, M.D. (2014) Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells. Optics Express, 22(2), pp. 1372-1380. (doi: 10.1364/OE.22.001372)

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Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

Item Type:Articles (Other)
Additional Information:This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.22.001372. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neale, Professor Steven
Authors: Jeorrett, A., Neale, S.L., Massoubre, D., Gu, E., Henderson, R.K., Millington, O., Mathieson, K., and Dawson, M.D.
Subjects:Q Science > QC Physics
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
ISSN (Online):1094-4087
Copyright Holders:Copyright © 2014 Optical Society of America
First Published:First published in Optics Express 22(2):1372-1380
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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