Genetic manipulation of toxoplasma gondii

Jacot, D., Meissner, M. , Sheiner, L. , Soldati-Favre, D. and Striepen, B. (2014) Genetic manipulation of toxoplasma gondii. In: Weiss, L.M. and Kim, K. (eds.) Toxoplasma Gondii. Elsevier Academic Press: Burlington, VT, pp. 577-611. ISBN 9780123965363 (doi: 10.1016/B978-0-12-396481-6.00017-9)

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The ability to genetically manipulate T. gondii enables reverse genetics and development of tools for genetic manipulation in the early 90's revolutionized the field. Currently T. gondii is the most genetically tractable apicomplexan parasite, with efficient methodology for both transient and stable transfection. In recent years, improved methods for regulated expression of genes, regulated destabilization of proteins, and efficient gene disruption have expanded the repertoire of powerful tools to genetically manipulate T. gondii. This chapter reviews the genetic tools available to investigators and provides a basic primer for common methods used in the laboratory for genetic studies of T. gondii.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Sheiner, Professor Lilach
Authors: Jacot, D., Meissner, M., Sheiner, L., Soldati-Favre, D., and Striepen, B.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Publisher:Elsevier Academic Press

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