Munday, J. C. et al. (2014) Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. Journal of Antimicrobial Chemotherapy, 69(3), pp. 651-663. (doi: 10.1093/jac/dkt442) (PMID:24235095) (PMCID:PMC3922157)
|
Text
87756.pdf - Published Version Available under License Creative Commons Attribution. 846kB |
Abstract
Objectives: Trypanosoma brucei drug transporters include the TbAT1/P2 aminopurine transporter and the high-affinity pentamidine transporter (HAPT1), but the genetic identity of HAPT1 is unknown. We recently reported that loss of T. brucei aquaglyceroporin 2 (TbAQP2) caused melarsoprol/pentamidine cross-resistance (MPXR) in these parasites and the current study aims to delineate the mechanism by which this occurs.<p></p> Methods: The TbAQP2 loci of isogenic pairs of drug-susceptible and MPXR strains of T. brucei subspecies were sequenced. Drug susceptibility profiles of trypanosome strains were correlated with expression of mutated TbAQP2 alleles. Pentamidine transport was studied in T. brucei subspecies expressing TbAQP2 variants.<p></p> Results: All MPXR strains examined contained TbAQP2 deletions or rearrangements, regardless of whether the strains were originally adapted in vitro or in vivo to arsenicals or to pentamidine. The MPXR strains and AQP2 knockout strains had lost HAPT1 activity. Reintroduction of TbAQP2 in MPXR trypanosomes restored susceptibility to the drugs and reinstated HAPT1 activity, but did not change the activity of TbAT1/P2. Expression of TbAQP2 sensitized Leishmania mexicana promastigotes 40-fold to pentamidine and >1000-fold to melaminophenyl arsenicals and induced a high-affinity pentamidine transport activity indistinguishable from HAPT1 by Km and inhibitor profile. Grafting the TbAQP2 selectivity filter amino acid residues onto a chimeric allele of AQP2 and AQP3 partly restored susceptibility to pentamidine and an arsenical.<p></p> Conclusions: TbAQP2 mediates high-affinity uptake of pentamidine and melaminophenyl arsenicals in trypanosomes and TbAQP2 encodes the previously reported HAPT1 activity. This finding establishes TbAQP2 as an important drug transporter.<p></p>
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and MacLeod, Professor Annette and De Koning, Professor Harry and Tait, Professor Andy and Turner, Professor Charles and Horn, Mr David and Munday, Dr Jane and Barrett, Professor Michael and Natto, Dr Manal and O'Connell, Dr Caroline |
| Authors: | Munday, J. C., Eze, A. A., Baker, N., Glover, L., Clucas, C., Aguinaga Andres, D., Natto, M. J., Teka, I. A., McDonald, J., Lee, R. S., Graf, F. E., Ludin, P., Burchmore, R. J. S., Turner, C. M. R., Tait, A., MacLeod, A., Mäser, P., Barrett, M. P., Horn, D., and De Koning, H. P. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Antimicrobial Chemotherapy |
| Publisher: | Oxford University Press |
| ISSN: | 0305-7453 |
| ISSN (Online): | 1460-2091 |
| Copyright Holders: | Copyright © 2013 The Authors |
| First Published: | First published in Journal of Antimicrobial Chemotherapy |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information