Human complement factor H. tissue specificity in the expression of three different mRNA species

Schwaeble, W., Schwaiger, H., Brooimans, R.A., Barbieri, A., Möst, J., Hirsch-Kauffmann, M., Tiefenthaler, M., Lappin, D.F., Daha, M.R. and Whaley, K. (1991) Human complement factor H. tissue specificity in the expression of three different mRNA species. European Journal of Biochemistry, 198(2), pp. 399-404. (doi: 10.1111/j.1432-1033.1991.tb16028.x)

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Abstract

Using cDNA clones H-19 and H-46, we have shown previously that three different mRNA species (4.3 kb, 1.8 kb and 1.4 kb) for complement factor H are expressed constitutively in human liver. Here we report data suggesting that the expression of these different factor-H mRNA species is regulated by tissue-specific control mechanisms. Total RNA and poly(A)-enriched RNA from various human tissues (heart, lung, temporal cortex, kidney, spleen, bone marrow and muscle) various cell lines (HepG2, HepG3, HepG4, Hep3B, H-4, Jurkat, Molt4, H-9, KHos24Os, A-431, U937, Mono Mac 6 and Raji) and from primary cultures of peripheral blood monocytes, fibroblasts and human umbilical vein endothelial cells (HUVEC) were investigated for the expression of factor-H mRNA. In RNA preparations from extrahepatic tissue, factor-H mRNA was only detected in biopsies from the lung. Using 20 micrograms total RNA isolated from all 13 cell lines it was not possible to detect any factor-H mRNA, while mRNA for factor H was expressed in monocytes, HUVEC and fibroblasts. When expressed in extrahepatic tissues, only the 4.3-kb and the 1.8-kb mRNA species were detected, while the 1.4-kb mRNA is expressed abundantly in liver. Interferon-gamma did not induce the expression of factor-H mRNA in any of the cell lines tested. On the other hand, tumour necrosis factor-alpha induced the expression of the 4.3-kb mRNA species in U937 cells. In HUVEC and fibroblasts the relative quantities of the 4.3-kb and the 1.8-kb mRNA species and the regulatory effects of interferon-gamma, interleukin-1, dexamethasone and retinoic acid on their expression showed significant tissue specificity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David
Authors: Schwaeble, W., Schwaiger, H., Brooimans, R.A., Barbieri, A., Möst, J., Hirsch-Kauffmann, M., Tiefenthaler, M., Lappin, D.F., Daha, M.R., and Whaley, K.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:European Journal of Biochemistry
ISSN:0014-2956
ISSN (Online):1432-1033

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