Davidson, A.J., King, J.S. and Insall, R.H. (2013) The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum. BioTechniques, 55(1), pp. 39-41. (doi: 10.2144/000114054)
Full text not currently available from Enlighten.
Abstract
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development. In addition, Dictyostelium-specific antibodies for other potential control proteins are not commercially available. Here we demonstrate the use of labeled streptavidin to detect biotinylated mitochondrial 3-methylcrotonyl-CoA carboxylase α (MCCC1), providing a robust and convenient tool for quantitative normalization of Dictyostelium Western blots, as well as fluorescently labeling mitochondria for microscopy of fixed cells.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | King, Dr Jason and Davidson, Mr Andrew and Insall, Professor Robert |
Authors: | Davidson, A.J., King, J.S., and Insall, R.H. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | BioTechniques |
ISSN: | 1940-9818 |
ISSN (Online): | 1940-9818 |
University Staff: Request a correction | Enlighten Editors: Update this record