Gibson, G.M. , Dienerowitz, M., Kelleher, P.A., Harvey, A.R. and Padgett, M.J. (2013) A multi-object spectral imaging instrument. Journal of Optics, 15(8), 085302. (doi: 10.1088/2040-8978/15/8/085302)
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84110.pdf - Published Version 1MB |
Abstract
We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gibson, Dr Graham and Padgett, Professor Miles and Dienerowitz, Dr Maria and Harvey, Professor Andy |
| Authors: | Gibson, G.M., Dienerowitz, M., Kelleher, P.A., Harvey, A.R., and Padgett, M.J. |
| College/School: | College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Journal of Optics |
| Publisher: | IOP Publishing Ltd. |
| ISSN: | 2040-8978 |
| ISSN (Online): | 2040-8986 |
| Copyright Holders: | Copyright © 2013 IOP Publishing Ltd. |
| First Published: | First published in the Journal of Optics 15(8):085302 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
| Data DOI: | 10.5525/gla.researchdata.158 |
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