Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline

Gabrielsen, M. , Riboldi-Tunnicliffe, A., Ibáñez-Shimabukuro, M., Griffiths, K., Roe, A.J. , Cooper, A., Smith, B. , Córsico, B. and Kennedy, M.W. (2012) Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 68, pp. 939-941. (doi: 10.1107/S1744309112026553) (PMID:22869127) (PMCID:PMC3412778)

Abstract

As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of `microdomains'. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kennedy, Professor Malcolm and Riboldi-Tunnicliffe, Dr Alan and Gabrielsen, Dr Mads and Roe, Professor Andrew and Smith, Dr Brian and Cooper, Professor Alan
• Authors: Gabrielsen, M., Riboldi-Tunnicliffe, A., Ibáñez-Shimabukuro, M., Griffiths, K., Roe, A.J., Cooper, A., Smith, B., Córsico, B., and Kennedy, M.W.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Science and Engineering > School of Chemistry
• Journal Name: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
• Publisher: International Union of Crystallography
• ISSN: 1744-3091
• ISSN (Online): 1744-3091
• Copyright Holders: Copyright © 2012 International Union of Crystallography
• First Published: First published in Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 68:939-941
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
46574	1	Structural and biophysical analysis of novel lipid binding proteins from parasitic helminths	Malcolm Kennedy	Wellcome Trust (WELLCOME)	083625/Z/07/Z	LS - ANIMAL BIOLOGY
49144	1	A biochemical and molecular analysis of the YhaO membrane protein in Escherichia coli O157:H7	Andrew Roe	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/G011389/1	III - BACTERIOLOGY