Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide

de Bont, H.B., Liskamp, R.M. , O'Brian, C.A., Erkelens, C., Veeneman, G.H. and van Boom, J.H. (1989) Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide. International Journal of Peptide and Protein Research, 33(2), pp. 115-123. (doi: 10.1111/j.1399-3011.1989.tb00196.x)

Abstract

The syntheses of a protein kinase C (PKC) peptide substrate, H-Lys-Arg-Thr-Leu-Arg-OH, and a phosphopeptide analog of the synthetic substrate, H-Lys-Arg-Thr(P)-Leu-Arg-OH, are reported. PKC phosphorylates the peptide with an apparent KM of 0.30 +/- 0.04 mM and an apparent Vmax equal to one-tenth that of histone III-S. The synthesis of the phosphopeptide features a recently developed convenient phosphorylation procedure for serine and threonine using N,N-diethylamino-dibenzylphosphoramidite. A complete characterization of the PKC substrate and its corresponding phosphopeptide by C-H COSY 2D n.m.r. is included.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Liskamp, Professor Robert
• Authors: de Bont, H.B., Liskamp, R.M., O'Brian, C.A., Erkelens, C., Veeneman, G.H., and van Boom, J.H.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: International Journal of Peptide and Protein Research
• ISSN: 0367-8377
• ISSN (Online): 1747-0285