Characterization and prognostic value of mutations in exons 5 and 6 of the p53 gene in patients with colorectal cancers in central Iran

Golmohammadi, R., Namazi, M.J., Nikbakht, M., Salehi, M. and Derakhshan, M.H. (2013) Characterization and prognostic value of mutations in exons 5 and 6 of the p53 gene in patients with colorectal cancers in central Iran. Gut and Liver, 7(3), pp. 295-302. (doi: 10.5009/gnl.2013.7.3.295)

Abstract

Background/Aims: We aimed to investigate the relation-ships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran. Methods: Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months. Results: Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p<0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p<0.01). Conclusions: Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Namazi, Dr Mohammad and Derakhshan, Professor Mohammad
• Authors: Golmohammadi, R., Namazi, M.J., Nikbakht, M., Salehi, M., and Derakhshan, M.H.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Gut and Liver
• Publisher: Gut and Liver, Editorial Office
• ISSN: 1976-2283
• ISSN (Online): 2005-1212
• Copyright Holders: Copyright © 2013 Korean Society of Gastroenterology et al.
• First Published: First published in Gut and Liver 7(3):295-302
• Publisher Policy: Reproduced under a Creative Commons License