TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection

Morrison, V.L. , Barr, T.A., Brown, S. and Gray, D. (2010) TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection. Journal of Immunology, 185(5), pp. 2737-2746. (doi: 10.4049/jimmunol.1000758) (PMID:20660707) (PMCID:PMC3745606)

Abstract

B cells recognize Ags on microorganisms both with their BCRs and TLRs. This innate recognition has the potential to alter the behavior of whole populations of B cells. We show in this study that in culture and in mice, MyD88-dependent activation of B cells via TLR2 or TLR9 causes the rapid loss of expression of CD62L by metalloproteinase-dependent shedding. Adoptive transfer of in vitro CpG-activated B cells showed them to be excluded from lymph nodes and Peyer’s patches, but not the spleen. In vivo, both injection of CpG and systemic infection with Salmonella typhimurium caused the shedding of CD62L and the consequent focusing of B cell migration to the spleen and away from lymph nodes. We propose that wholesale TLR-mediated changes to B cell migration influence the development of immunity to pathogens carrying appropriate ligands.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Barr, Dr Tom and Morrison, Dr Vicky
• Authors: Morrison, V.L., Barr, T.A., Brown, S., and Gray, D.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Immunology
• ISSN: 0022-1767