Warren, R.L., Tassieri, M. , Li, X., Glidle, A., Paterson, D.J., Carlsson, A. and Cooper, J.M. (2013) Rheology at the micro-scale: new tools for bio-analysis. In: Optical Methods for Inspection, Characterization and Imaging of Biomaterials, Munich, Germany, 13-16 May 2013, (doi: 10.1117/12.2025254)
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Publisher's URL: http://dx.doi.org/10.1117/12.2025254
Abstract
We present a simple and non-invasive experimental procedure to measure the linear viscoelastic properties of cells by passive particle tracking microrheology. In order to do this, a generalised Langevin equation is adopted to relate the timedependent thermal fluctuations of a probe sensor, immobilised to the cell’s membrane, to the frequency-dependent viscoelastic moduli of the cell. The method has been validated by measuring the linear viscoelastic response of a soft solid and then applied to cell physiology studies. It is shown that the viscoelastic moduli are related to the cell’s cytoskeletal structure, which in this work is modulated either by inhibiting the actin/myosin-II interactions by means of blebbistatin or by varying the solution osmolarity from iso- to hypo-osmotic conditions. The insights gained from this form of rheological analysis promises to be a valuable addition to physiological studies; e.g. cell physiology during pathology and pharmacological response.
| Item Type: | Conference Proceedings |
|---|---|
| Additional Information: | Copyright 2013 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2025254 |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Warren, Ms Rebecca and Tassieri, Dr Manlio and Glidle, Dr Andrew and Carlsson, Dr Allan and Cooper, Professor Jonathan |
| Authors: | Warren, R.L., Tassieri, M., Li, X., Glidle, A., Paterson, D.J., Carlsson, A., and Cooper, J.M. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| ISSN: | 0277786X |
| Copyright Holders: | Copyright © 2013 SPIE |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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