Vance, S. J., McDonald, R. E., Cooper, A., Kennedy, M. W. and Smith, B. O. (2014) Resonance assignments for latherin, a natural surfactant protein from horse sweat. Biomolecular NMR Assignments, 8(1), pp. 213-216. (doi: 10.1007/s12104-013-9485-3)
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Publisher's URL: http://dx.doi.org/10.1007/s12104-013-9485-3
Abstract
Latherin is an intrinsically surfactant protein of ~23 kDa found in the sweat and saliva of horses. Its function is probably to enhance the translocation of sweat water from the skin to the surface of the pelt for evaporative cooling. Its role in saliva may be to enhance the wetting, softening and maceration of the dry, fibrous food for which equines are adapted. Latherin is unusual in its relatively high content of aliphatic amino acids (~25 % leucines) that might contribute to its surfactant properties. Latherin is related to the palate, lung, and nasal epithelium carcinoma-associated proteins (PLUNCs) of mammals, at least one of which is now known to exhibit similar surfactant activity to latherin. No structures of any PLUNC protein are currently available. 15N,13C-labelled recombinant latherin was produced in Escherichia coli, and essentially all of the resonances were assigned despite the signal overlap due to the preponderance of leucines. The most notable exceptions include a number of residues located in an apparently dynamic loop region between residues 145 and 154. The assignments have been deposited with BMRB accession number 19067.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Kennedy, Professor Malcolm and Smith, Dr Brian and Cooper, Professor Alan |
| Authors: | Vance, S. J., McDonald, R. E., Cooper, A., Kennedy, M. W., and Smith, B. O. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Chemistry College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | Biomolecular NMR Assignments |
| Publisher: | Springer |
| ISSN: | 1874-2718 |
| ISSN (Online): | 1874-270X |
| Copyright Holders: | Copyright © 2013 The Authors |
| First Published: | First published in Biomolecular NMR Assignments 8(1):213-216 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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