Transfection of rodent malaria parasites

Philip, N., Orr, R. and Waters, A.P. (2013) Transfection of rodent malaria parasites. Methods in Molecular Biology, 923, pp. 99-125.

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Abstract

Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.

Item Type:Articles
Additional Information:ISBN: 9781627030250
Status:Published
Refereed:No
Glasgow Author(s) Enlighten ID:Cameron, Mrs Rachael and Waters, Professor Andy and Philip, Dr Nisha
Authors: Philip, N., Orr, R., and Waters, A.P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Methods in Molecular Biology
ISSN:1064-3745

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