Transfection of rodent malaria parasites

Philip, N., Orr, R. and Waters, A.P. (2013) Transfection of rodent malaria parasites. Methods in Molecular Biology, 923, pp. 99-125.

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Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.

Item Type:Articles
Additional Information:ISBN: 9781627030250
Glasgow Author(s) Enlighten ID:Cameron, Mrs Rachael and Philip, Dr Nisha and Waters, Professor Andy
Authors: Philip, N., Orr, R., and Waters, A.P.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Methods in Molecular Biology

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