Roussakis, E., Voutsadaki, S., Pinakoulaki, E., Sideris, D.P., Tokatlidis, K. and Katerinopoulos, H.E. (2008) ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions. Cell Calcium, 44(3), pp. 270-275. (doi: 10.1016/j.ceca.2007.12.008)
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Abstract
A new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3′,2′:3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Tokatlidis, Professor Kostas |
| Authors: | Roussakis, E., Voutsadaki, S., Pinakoulaki, E., Sideris, D.P., Tokatlidis, K., and Katerinopoulos, H.E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Cell Calcium |
| ISSN: | 0143-4160 |
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